重组蛋白表达
Recombinant Protein Expression

苏州智享重组蛋白表达平台可利用成熟的工艺流程确保快速交付高质量的重组蛋白，帮助客户更快地推动科研项目和候选药物的进展。苏州智享团队拥有十年以上的专业蛋白表达纯化经验，能为客户提供mg至g级的高纯度活性蛋白定制服务，可以有效地节约您的经济成本和时间成本。

IntellectiveBio recombinant protein expression platform has a mature process, which ensures that we can quickly deliver high-quality recombinant proteins, helping customers promote projects, and shorten the time for drug candidate screening. IntellectiveBio team has more than ten years of experience in expression and purification. We can provide customized services of high-purity active proteins in the mg to g range, effectively saving your economic and time costs.

选择智享的理由:
Why IntellectiveBio?
  • 一站式技术服务：从结构解析、序列优化、载体构建、宿主选择，再到表达制备、蛋白纯化、分析鉴定、质量检测，最后提供完整实验报告，我们可以根据客户的实际使用需求提供全流程定制化服务
  • One-stop technical services: from structure analysis, sequence optimization, carrier construction, host selection, expression preparation, protein purification, analysis and identification, quality testing, and finally to provide a complete experimental report, we can provide full-process customized services according to the actual use needs of customers
  • 多样化蛋白表达系统满足不同的表达需求，包括：
    Diverse protein expression systems meet different expression needs, including:
    • 原核蛋白表达系统：大肠杆菌等
    • Prokaryotic protein expression system: E.coli, etc
    • 哺乳动物表达系统：CHO, HEK293等
    • Mammalian expression system: CHO, HEK293, etc
    • 营养缺陷型细胞株：DHFR, GS等
    • Dystrophic cell lines: DHFR, GS, etc
  • 可提供大规模无血清、悬浮培养生产服务，规模可达200L
  • Large-scale serum-free, suspension culture production with the maximum size up to 200L
哺乳动物细胞表达系统
Mammalian Cell Expression System

哺乳动物细胞表达系统有最接近于人类蛋白的翻译后修饰，在蛋白表达过程中会形成接近人类蛋白的折叠和聚合，使其具备活性蛋白所必须的空间结构和修饰。哺乳动物细胞表达系统在活性方面远胜于原核表达系统及酵母、昆虫细胞等真核表达系统。 苏州智享技术团队拥有丰富的CHO及HEK293细胞表达经验，可提供标签切除、内毒素去除、表达量优化等多种服务。

Mammalian cell expression systems have the closest post-translational modification as human proteins. In the process of protein expression, the folding and polymerization mode close to human protein helps forming the necessary spatial structure and modifications essential for biological activities. In terms of activity, mammalian cell expression system is far superior to prokaryotic expression systems and eukaryotic expression systems such as yeast and insect cells. Our technical team has rich experience in the expression of CHO and HEK293 cells, and can provide various services including tag removal, endotoxin removal, expression optimization etc.

服务优势
Our Advantages
  • 我们的载体可在哺乳动物细胞中高表达重组蛋白，表达量最高可达1-3g/L。
  • Mammalian cells with our vector can highly express recombinant protein with an expression level up to 1-3g/L
  • 哺乳动物细胞表达成功率高达95%
  • 95% success rate of mammalian cell expression system
  • 提供CHO、Expi293和ExpiCHO等细胞瞬时蛋白表达服务
  • Transient protein expression service of CHO, Expi293 and ExpiCHO host cells